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KMID : 1005220080230030177
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Journal of Embryo Transfer
2008 Volume.23 No. 3 p.177 ~ p.181
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Effects of hCG Treatment on the Pregnancy Rates and Progesterone Concentrations in Hanwoo Recipients with SCNT Embryos
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Hwang Seong-Soo
Yang Byoung-Chul
Im Gi-Sun
Ko Yeoung-Gyu
Choi Sun-Ho
Min Kwan-Sik
Yoon Jong-Taek
Seong Hwan-Hoo
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Abstract
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This study was performed to investigate the effects of hCG treatment on pregnancy and delivery rates in the Hanwoo recipients. There were significantly higher pregnancy and delivery rates in the recipients treated with hCG at 7 days after artificial insemination (p<0.05), respectively. The SCNT embryos from bovine fetal fibroblast cells were transferred into the synchronized recipients. The recipients were administered saline (n=89) or hCG (1,500 IU) (n=48) at 7 days after heat, respectively. The pregnancy rate was significantly higher in the recipients treated with hCG compared to that of saline treated group (p<0.01), however, the delivery rate was not different in both treated groups. The concentration of plasma progesterone (P4) was not different in both groups before hCG treatment, but the P4 level was increased significantly in hCG treated group after hCG injection (p<0.05). Although the pregnancy rate was very high in early stage of pregnancy, it was decreased dramatically after 50 days of pregnancy and maintained basal level. Taken together, the treatment of hCG in the SCNT recipients after day 7 of heat was effective method to increase the P4 concentration and to increase the pregnancy rate. But it did not affect directly to delivery.
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KEYWORD
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hCG, pregnancy, delivery, progesterone, SCNT
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